STRyper

STRyper analyzes microsatellite markers via capillary sequencing for genotyping and chromatogram management.

Key Features:

• Chromatogram import and management: STRyper imports and manages chromatograms from FSA and HID files with search capabilities including smart folders.
• Size standard and marker panel creation: STRyper creates size standards and microsatellite marker panels including bins.
• Manual correction capabilities: STRyper allows manual correction of DNA ladders and individual genotypes by drag-and-drop.
• Electrophoretic variation mitigation: STRyper introduces a new method to mitigate the effect of variations in electrophoretic conditions on estimated allele sizes.

Scientific Applications:

• Microsatellite genotyping: STRyper performs genotyping of microsatellite markers for rapid genotyping and low-cost studies across species beyond human genotyping.
• Capillary sequencing analysis: STRyper analyzes chromatograms from capillary sequencing for microsatellite marker studies.

Methodology:

STRyper uses macOS libraries and processes chromatograms imported from FSA and HID files.