SwePep

SwePep provides a curated database of 4,180 annotated endogenous peptides from 394 species, including 50 novel peptides identified in brain tissue, to enable rapid identification of endogenous peptides in complex tissue samples by mass spectrometry.

Key Features:

• Comprehensive Database: Contains 4,180 annotated endogenous peptides derived from various tissues across 394 species and includes 50 novel peptides identified in brain tissue.
• Post-Translational Modifications (PTMs): Supports comparison of experimental peptide masses with theoretical masses stored in the database while considering potential PTMs to improve identification accuracy.
• Relational Database Structure: Implemented with MySQL and Java to manage and retrieve peptide attributes including mass, isoelectric point, sequence, and precursor protein information.

Scientific Applications:

• Peptidomics: Accelerates mass spectrometry-based identification of endogenous peptides to support peptidomics studies.
• Novel peptide discovery: Supports discovery and annotation of novel endogenous peptides, as exemplified by the 50 novel brain peptides included in the database.
• Functional peptide surveys: Enables comprehensive surveys of endogenous peptides to advance understanding of their roles in biological processes.

Methodology:

SwePep employs a two-step identification approach in which experimental peptide masses are compared against database entries and theoretical masses both with and without potential PTMs to narrow candidate peptides, followed by confirmation using tandem mass spectrometry (MS/MS) data.