SynBioStrainFinder

SynBioStrainFinder provides a curated database of microbial strain information focusing on CRISPR/Cas gene-editing systems and related cultivation methods, genome sequences, and compound associations to support strain selection and engineering.

Key Features:

• Strain2Gms/PredStrain2Gms: Contains 1,426 manually curated CRISPR/Cas gene-editing records from 157 microbial strains and offers PredStrain2Gms recommendations for strains lacking established CRISPR/Cas systems based on closely related strains.
• Strain2BasicInfo: Aggregates cultivation protocols and genome sequence records totaling 139,499 entries for microbial strains.
• Strain2Compd: Links 773,298 compound-related records to strains to support metabolic and bioproduct analyses.
• Star Labeling System: Annotates strains with star labels reflecting the comprehensiveness and availability of associated information.

Scientific Applications:

• Gene-editing system development: Provides curated CRISPR/Cas records and predicted recommendations to guide construction of gene-editing systems in target strains.
• Strain engineering and metabolic research: Integrates cultivation methods, genome sequences, and compound associations to support genetic manipulation and metabolic pathway analysis.
• Bioproduct and pathway discovery: Enables investigation of strain–compound relationships for metabolite identification and bioproduct development.

Methodology:

Manual extraction and curation of CRISPR/Cas gene-editing records from scientific literature, integration of cultivation methods, genome sequences, and compound-related data, and PredStrain2Gms recommendations based on closely related strains.