SynergyFinder plus (SynergyFinder+)

SynergyFinder plus (SynergyFinder+) analyzes drug combination dose–response data to quantify and visualize synergistic and antagonistic interactions for rational design of combination therapies.

Key Features:

• Multiple Synergy Scoring Models: Implements HSA (Highest Single Agent), Loewe, Bliss, and ZIP models to evaluate interactions between drug pairs across dose–response matrices.
• Dose–Response Matrix Analysis: Performs systematic evaluation of drug pairs across concentration matrices to compute synergy scores and interaction patterns.
• Synergy Landscape Visualization: Provides two-dimensional and three-dimensional visualization of synergy scores over dose matrices to represent spatial patterns of interaction.
• Integration with Automated Screening: Integrates automated screening techniques with synergy scoring to support analysis of high-throughput combination experiments.

Scientific Applications:

• Cancer combination therapy development: Identifies and prioritizes drug combinations that selectively inhibit cancer cell viability and growth while addressing resistance mechanisms.
• High-throughput drug combination screening: Supports analysis of large-scale combination screens to detect synergistic interactions across concentration ranges.
• Study of drug sensitivity and resistance: Characterizes variability in treatment responses and resistance mechanisms using synergy scoring models.

Methodology:

An experimental–computational pipeline integrates automated screening techniques with HSA, Loewe, Bliss and ZIP synergy scoring algorithms to detect synergistic drug interactions within specific concentration windows.