TADMaster

TADMaster evaluates and compares topologically associated domains (TADs) to assess concordance, identify consensus and unique domains, and characterize structural variation across genomic regions and experimental conditions.

Key Features:

• Concordance Evaluation: Allows direct assessment of agreement between two or more TAD datasets within a specified genomic region.
• Visual and Quantitative Metrics: Provides visualizations and quantitative metrics for comparing domain numbers, sizes, shared domains, boundaries, and overlaps.
• Consensus and Unique Domain Identification: Derives consensus and unique TADs from multiple datasets to aid selection of reliable domains for further analysis.

Scientific Applications:

• Chromatin organization and gene regulation: Facilitates analysis of how TAD architecture influences chromosomal architecture and gene regulation across developmental stages and disease states.
• Comparative analysis across cell lines and conditions: Enables robust comparison of TADs identified from different cell lines or experimental conditions to identify consensus and variable domains.

Methodology:

Processes and analyzes chromosome conformation capture data and its derivatives and evaluates concordance and robustness of TAD datasets using quantitative metrics and visualizations.