TagGD

TagGD generates DNA barcodes and demultiplexes multiplexed next-generation sequencing (NGS) data to maximize sample throughput while tolerating sequencing errors.

Key Features:

• Customizable Barcode Generation: Generates thousands of unique barcodes that adhere to user-defined constraints.
• Error-aware Design: Accounts for insertion, deletion, and substitution errors during barcode design to minimize experimental interference.
• High-speed Performance: Capable of designing 20,000 barcodes of length 18 within five minutes.
• Accurate Demultiplexing: Maintains full demultiplexing accuracy at error rates up to 2% and can process up to 2 million barcoded Illumina HiSeq-like reads within five minutes.
• Multiplexing Scalability: Supports extensive multiplexing where sample abundance allows, enabling high-throughput experiments.

Scientific Applications:

• Genomics: Enables multiplexed NGS for simultaneous sequencing of many genomic samples.
• Transcriptomics: Supports multiplexed RNA-seq experiments to increase sample throughput.
• Metagenomics: Facilitates large-scale multiplexed metagenomic sequencing of complex communities.

Methodology:

Uses an algorithm that explicitly accounts for insertion, deletion, and substitution errors during barcode design and demultiplexing to enable rapid processing of large datasets.