TALE-NT

TALE-NT predicts and designs TAL effector binding specificities to enable custom TAL effector repeat array construction and genome-wide binding-site identification for genome targeting and analysis of plant–pathogen interactions.

Key Features:

• Custom TAL effector repeat array design: Designs bespoke TAL effector repeat sequences tailored to specified DNA targets using the TAL effector–nucleotide binding code.
• Prediction of TAL effector binding sites: Searches genomes, promoteromes, or user-specified sequences to predict potential TAL effector binding sites.
• Ranking of predicted sites: Ranks predicted binding sites by likelihood to prioritize probable binding locations.
• Configurable architecture and search parameters: Supports configurable search parameters to accommodate different TAL effector and TAL effector nuclease (TALEN) architectures.
• Off-target identification: Predicts potential off-target interactions to assist in minimizing unintended effects during gene editing.

Scientific Applications:

• Custom genome editing tools: Design of TAL effector repeat arrays and TALENs for targeted genome editing and construction of custom transcriptional regulators.
• Plant–pathogen interaction studies: Identification of effector target sites to study host gene targets and mechanisms of plant disease.
• Off-target assessment for precision engineering: Evaluation of potential off-target sites to improve specificity of gene editing experiments.
• Synthetic biology and gene regulation: Development of targeted transcription factors for controlled regulation of gene expression.

Methodology:

Uses the TAL effector–nucleotide binding code to map repeat domains to nucleotides; designs bespoke repeat arrays for specified targets; searches genomes, promoteromes, or user-specified sequences for potential binding sites; ranks predictions by likelihood; supports configurable search parameters for different TAL effector and TALEN architectures; predicts potential off-target interactions.