TALENoffer

TALENoffer predicts genome-wide TALEN off-target sites to assess potential unintended genomic cleavage.

Key Features:

• Genome-wide prediction: Scans entire mammalian genomes to identify potential TALEN off-target binding and cleavage sites.
• Off-target identification accuracy: Recovers known off-targets for engineered TALENs, demonstrating predictive accuracy against validated sites.
• Runtime performance: Scans complete mammalian genomes within a few minutes, enabling rapid prediction throughput.

Scientific Applications:

• Genome editing precision assessment: Enables assessment and mitigation of off-target effects in TALEN-mediated genome editing experiments.
• Functional genomics: Identifies potential unintended modifications that could confound functional genomics studies.
• Gene therapy development: Informs design and safety assessment of TALEN-based gene therapies by highlighting candidate off-target sites.
• Synthetic biology: Supports design of synthetic constructs by predicting unintended TALEN interactions with the genome.

Methodology:

Performs genome-wide scans of mammalian genomes to predict potential TALEN off-target binding and cleavage, validates predictions by recovering known engineered TALEN off-targets, and is optimized for runtime to scan complete mammalian genomes within minutes.