TarBase v8

TarBase v8 catalogs experimentally validated microRNA (miRNA) targets and provides curated details to support research on miRNA–gene regulatory interactions.

Key Features:

• Extensive data collection: Contains over 1 million entries representing approximately 670,000 unique miRNA–target pairs.
• Diverse experimental support: Records interactions supported by more than 33 experimental methodologies across ~600 cell types/tissues and ~451 experimental conditions.
• Detailed interaction annotation: Each entry includes miRNA- and gene-related facts, specific interaction details, experimental validation methodologies, and outcomes, together with function-related data.
• Target prediction integration: DIANA microT miRNA target prediction scores and related prediction details are associated with interactions.
• External database integration: Interaction entries are enriched with general information imported from UniProt, Ensembl, and RefSeq.
• Evidence annotation: Interactions are annotated for the type and robustness of supporting experimental methodologies, including positive and negative validation outcomes.

Scientific Applications:

• Regulatory network analysis: Supports mapping and analysis of miRNA–gene regulatory interactions across biological contexts and experimental conditions.
• miRNA functional studies: Enables investigation of miRNA roles and functions through curated, experimentally supported target relationships.
• Disease mechanism and therapeutic development: Provides experimentally validated targets useful for studies of disease mechanisms and for identifying potential therapeutic targets.

Methodology:

Entries are manually curated and annotated with experimentally supported methodologies and outcomes; DIANA microT prediction scores are attached to interactions and general annotations are integrated from UniProt, Ensembl, and RefSeq.