TARDIS

TARDIS automates the integration and quality assessment of chromatographic peaks in targeted LC-MS metabolomics and lipidomics data.

Key Features:

• Peak Integration: Automated chromatographic peak integration for targeted LC-MS data.
• Quality Metrics: Includes established metrics (area under the curve, maximum intensity, points over the peak) and recently developed metrics (custom signal-to-noise ratio, bell-curve similarity) for peak quality evaluation.
• Cross-Platform Validation: Demonstrated robustness through quantitative comparison to state-of-the-art vendor software across three biofluids (stool, saliva, urine) and two LC-MS instruments.
• Scalability: Validated at large scale (n = 1786) using data from the FAME, ENVIRONAGE, and FGFP cohort studies.

Scientific Applications:

• Targeted Metabolomics: Automated preprocessing and analysis of targeted LC-MS metabolomics data.
• Targeted Lipidomics: Automated preprocessing and analysis of targeted LC-MS lipidomics data.
• Multi-Biofluid Analysis: Applicable to diverse biological matrices including stool, saliva, and urine.

Methodology:

TARDIS implements automated chromatographic peak integration with quality assessment using established metrics (area under the curve, maximum intensity, points over the peak) and recently developed metrics (custom signal-to-noise ratio, bell-curve similarity).