TASUKE

TASUKE visualizes large-scale resequencing data generated by next-generation sequencing by displaying sequence variations and read depth across multiple genomes.

Key Features:

• Simultaneous Visualization: Displays variants and read-depth information for multiple genomes concurrently at various scales.
• Scalable Display: Supports variable block sizes ranging from 1 bp to 100 kb for multiscale inspection.
• Color-Coded Representation: Encodes variant frequency and coverage depth using gradational colors within variable-sized blocks.
• Annotation Overlay: Integrates genomic annotations with resequencing data for combined interpretation.
• Large-Scale Dataset Support: Designed to handle extensive resequencing datasets from many samples.

Scientific Applications:

• Comparative Genomics: Enables visualization of conserved and divergent regions across multiple genomes.
• Population Genetics: Facilitates analysis of variant frequencies and coverage depth across populations.
• Functional Genomics Annotation: Supports integration of annotation data with sequence variation and coverage to interpret genomic features.

Methodology:

Demonstrated using 50 rice and 100 human genome resequencing datasets.