TeaAS

TeaAS provides a curated database and analytical resource for alternative splicing (AS) events in the tea plant Camellia sinensis.

Key Features:

• Comprehensive Data Integration: Integrates genome annotation, AS event classification, transcript sequences, and isoform expression levels derived from 66 RNA-seq datasets totaling 3.96 terabytes of reads.
• Flexible Retrieval Methods: Enables retrieval by Gene ID, gene name, annotation (non-redundant, Kyoto Encyclopedia of Genes and Genomes (KEGG), or Gene Ontology), chromosomal location, and RNA-seq dataset.
• Environmental and Tissue-Specific Analysis: Identifies AS events occurring under different environmental conditions and across varied tissue types.
• Expression Level Insights: Provides expression-level information for specific transcripts and isoforms to support analysis of AS-mediated gene regulation.
• Bioinformatics Tools: Includes Basic Local Alignment Search Tool (BLAST) for sequence alignment and a Generic Genome Browser for visualization of gene structure and AS event mapping.

Scientific Applications:

• Plant growth, development and stress responses: Supports analysis of the molecular roles of alternative splicing in growth, development, and stress tolerance in Camellia sinensis.
• Woody crop comparative studies: Serves as a reference platform for investigating alternative splicing in woody crops and for identifying novel biological functions and regulatory mechanisms.

Methodology:

Integrates genome annotation, classified AS events, transcript sequences, and isoform expression derived from 66 RNA-seq datasets (3.96 TB), and provides BLAST for sequence alignment and a Generic Genome Browser for visualization.