TELCoMB

TELCoMB elucidates resistome and mobilome composition and diversity and uncovers ARG-MGE colocalizations in microbial communities through a Snakemake workflow that improves sensitivity for detecting antimicrobial resistance genes and mobile genetic elements.

Key Features:

• Resistome and Mobilome Analysis: Analyzes antimicrobial resistance genes and mobile genetic elements in microbial communities.
• ARG-MGE Colocalization Detection: Uncovers colocalizations between antimicrobial resistance genes and mobile genetic elements.
• Multi-Platform Sequencing Support: Supports both short-read and long-read sequencing data without requiring enrichment.
• Output Generation: Generates publication-ready figures and CSV files for comprehensive analysis.

Scientific Applications:

• Resistome Characterization: Identifies and characterizes antimicrobial resistance genes within microbial populations.
• Mobilome Analysis: Detects mobile genetic elements harboring antimicrobial resistance genes.
• ARG-MGE Association Studies: Reveals genetic linkages between resistance genes and mobile elements to understand resistance spread mechanisms.
• Antimicrobial Resistance Surveillance: Improves sensitivity of detection for rare antimicrobial resistance genes and mobile genetic elements in complex microbial communities.

Methodology:

TELCoMB is implemented as a Snakemake workflow. The protocol includes data preprocessing, calculation of resistome distribution and composition, and identification of ARG-MGE colocalizations.