TEMM

TEMM catalogs transposon element-based molecular markers (TE-markers), associated primer sequences, and PCR protocols to support studies of plant genetic diversity, phylogeny, and breeding.

Key Features:

• Categorization of TE-markers: TEMM distinguishes between retrotransposon-based markers (RBIP, IRAP, REMAP, iPBS) and DNA-based transposon markers (MITE, TE-junction, CACTA TE-markers) based on their transcription mechanisms.
• Data compilation: TEMM contains 784 primer sequences with their associated PCR reaction protocols for amplifying transposable elements in plant genomes.
• Cross-crop applicability: The resource documents the application of TE-markers across various crops for genetic, phylogenetic, and evolutionary analyses.
• Breeding support: Provided primer sequences and PCR protocols facilitate marker-based approaches in breeding for agronomic traits such as yield, disease resistance, and stress tolerance.

Scientific Applications:

• Genetic Diversity Assessment: Use of TE-marker variation to quantify genetic diversity within and between plant populations.
• Phylogenetic Analysis: Use of TE-markers to infer evolutionary relationships and construct phylogenetic trees among plant species.
• Evolutionary Biology: Use of TE-markers to investigate the evolutionary history of plant genomes.
• Breeding Programs: Integration of TE-marker data into marker-assisted breeding to improve agronomic traits such as yield, disease resistance, and stress tolerance.

Methodology:

Compilation and curation of 784 primer sequences and their associated PCR reaction protocols.