emalign

emalign aligns three-dimensional density maps in cryo-electron microscopy by exploiting common lines between projection images of the maps.

Key Features:

• Fully automatic alignment: The algorithm automatically handles rotations, reflections (handedness), and translations between density maps.
• Symmetry-agnostic operation: The algorithm is applicable to any type of molecular symmetry without requiring any information regarding the symmetry of the maps.
• Accuracy and efficiency: The alignment algorithm demonstrates accuracy and efficiency on publicly available density maps.

Scientific Applications:

• Density map comparison: Aligning three-dimensional density maps of macromolecules for comparison in cryo-electron microscopy data processing.

Methodology:

The algorithm exploits common lines between projection images of the density maps.