TigerFISH

TigerFISH designs oligonucleotide probes for genome-scale targeting of repetitive DNA intervals for fluorescent in situ hybridization (FISH).

Key Features:

• Genome-scale probe design: Designs oligonucleotide probes against highly repetitive DNA intervals across entire genomes.
• Interval-specific repeat probes: Creates panels of probes specific to distinct repetitive DNA intervals.
• Chromosome-specific targeting: Enables design of repeat probes specific to individual chromosomes.

Scientific Applications:

• Visualization of repetitive regions: Enables targeted visualization of repetitive DNA regions in their native chromosomal contexts via FISH.
• Metaphase spread imaging: Supports imaging of repetitive DNA probes on metaphase chromosome spreads.
• Interphase nucleus imaging: Supports imaging of repetitive DNA probes in interphase nuclei.
• Highly multiplexed FISH assays: Extends oligo-based FISH capabilities to include highly repetitive DNA as targets in multiplexed experiments.

Methodology:

TigerFISH uses computational design of oligonucleotide probes to target intervals of highly repetitive DNA at genome scale.