TIsigner

TIsigner optimizes translation initiation coding regions, identifies soluble protein domains, and predicts signal peptides to improve recombinant protein expression, solubility, and subcellular targeting using prediction algorithms.

Key Features:

• TIsigner (Translation Initiation coding region designer): Performs synonymous optimization of the translation initiation coding region to enhance translation efficiency and protein expression.
• SoDoPE (Soluble Domain for Protein Expression): Identifies soluble domains within proteins to guide solubility optimization and improve folding of expressed proteins.
• Razor: Predicts optimal signal peptides to direct proteins to correct cellular locations and support proper processing.
• Integrated workflow: Enables combined use of translation-initiation optimization, soluble-domain identification, and signal peptide prediction for coordinated gene optimization.

Scientific Applications:

• Recombinant protein production optimization: Improve yields by optimizing translation initiation coding regions and sequence synonyms.
• Protein solubility enhancement: Increase likelihood of obtaining soluble, correctly folded proteins by selecting soluble domains.
• Subcellular targeting and processing: Aid correct localization and processing by predicting appropriate signal peptides.
• Sequence-level design for expression experiments: Guide genetic modifications at the coding-region and domain levels to refine expression outcomes.

Methodology:

Computational methods explicitly include synonymous optimization of the translation initiation coding region, soluble-domain identification for solubility analysis, and prediction of signal peptides using prediction algorithms.