TockyPrep

TockyPrep automates data preprocessing of Fluorescent Timer reporter data from flow cytometry experiments for quantitative single-cell analysis, standardizing Timer data analysis to enhance reproducibility and accuracy.

Key Features:

• Trigonometric Transformation: Implements a trigonometric transformation method to elucidate the dynamics of Fluorescent Timer proteins.
• Fluorescence Normalization: Normalizes immature and mature Timer fluorescence data as essential preprocessing for robust analysis of Timer maturation dynamics.
• Basic Visualization: Provides tools for basic visualization of Timer fluorescence data.

Scientific Applications:

• Temporal Dynamics Analysis: Analyzes time-dependent changes in emission spectra of Fluorescent Timer proteins at the single-cell level.
• Tocky Tool Integration: Enhances experimental systems utilizing Fluorescent Timer proteins, including the Timer-of-cell-kinetics-and-activity (Tocky) tools with Fast-FT.
• Cross-Experimental Standardization: Enables reproducible and accurate Timer data analysis across different flow cytometry instrument settings and experimental setups.

Methodology:

TockyPrep implements trigonometric transformation of Timer fluorescence data and normalization of immature and mature Timer fluorescence signals.