TOGGLE

TOGGLE provides a modular toolbox for constructing customizable pipelines to process Next-Generation Sequencing (NGS) data and enable analyses such as single nucleotide polymorphism (SNP) discovery.

Key Features:

• Modular Design: Provides a modular framework for assembling customizable analysis pipelines.
• Single configuration file: Centralizes pipeline parameters and experimental settings in a single basic configuration file.
• Pipeline Management: Orchestrates integration of multiple NGS tools and manages processing from raw FASTQ files to biologically relevant outputs.
• Scalability: Supports deployment across individual workstations up to High-Performance Computing (HPC) clusters.
• Reliability and Logging: Implements robust log tracking and data flow management to support reproducibility and data integrity.

Scientific Applications:

• SNP discovery: Enables end-to-end analysis pipelines for single nucleotide polymorphism (SNP) discovery across large genomic datasets.

Methodology:

Construction of modular pipelines by integrating a wide array of NGS tools, driven by a single basic configuration file, managing data flow from raw FASTQ to biologically relevant outputs, with logging and support for deployment from workstations to HPC clusters.