TOMOJ

TOMOJ performs transmission electron tomography reconstruction and analysis to generate three-dimensional representations of cellular and subcellular structures.

Key Features:

• ImageJ plug-in: Implemented as a plug-in for ImageJ for processing transmission electron tomography (ETT) data.
• Tomogram alignment: Aligns projection series to prepare datasets for volume reconstruction.
• Volume reconstruction algorithms: Implements algebraic reconstruction technique (ART), simultaneous iterative reconstruction technique (SIRT), and weighted back-projection for tomographic reconstruction.
• Multi-volume integration: Aligns and integrates multiple tomographic volumes to produce combined three-dimensional reconstructions.
• Three-dimensional representation: Produces accurate three-dimensional reconstructions suitable for structural analysis of cellular components.

Scientific Applications:

• Transmission electron tomography (ETT): Reconstruction and structural analysis of specimens imaged by transmission electron microscopy.
• Subcellular structural analysis: Investigation of subcellular components and cellular architecture at intermediate resolution.
• Complementary structural methods: Provides intermediate-resolution 3D data that complements high-resolution techniques such as X-ray diffraction and nuclear magnetic resonance (NMR) and traditional optical microscopy.
• Cell, molecular, and structural biology: Applicable to studies in cell biology, molecular biology, and structural biology requiring ultrastructural three-dimensional information.

Methodology:

Performs tomogram alignment followed by volume reconstruction using ART, SIRT, and weighted back-projection, and supports alignment/integration of multiple tomographic volumes.