Toolyard Bio

Free collection of 67 DNA and protein sequence tools and 44 lab calculators that run in the web browser, including reverse complement, translation, ORF finder, restriction maps, pairwise alignment, primer Tm, CRISPR guide finder, codon optimization, plasmid maps, and molarity, dilution, qPCR ΔΔCt and IC50 calculators. Sequences are processed on the user's device and never uploaded.

Topics

Details

License:
Freeware
Maturity:
Mature
Cost:
Free of charge
Tool Type:
web application
Operating Systems:
Linux, Mac, Windows
Added:
10/5/2026
Last Updated:
10/5/2026

Operations

Data Inputs & Outputs

Codon usage analysis

Outputs

    PCR primer design

    Outputs

      Restriction site recognition

      Outputs

        Sequence composition calculation