Topino

Topino transforms molecular-stream separation (MSS) image data into angulagrams and quantifies stream width, stream linearity, and stream deflection to enable quantitative assessment of molecular separations.

Key Features:

• Data type handling: Processes MSS data comprising intensity measurements with two spatial coordinates (three-dimensional intensity + spatial data).
• Polar coordinate transformation: Transforms MSS data into a polar coordinate system.
• Radial integration (convolution): Integrates intensity along the radius axis to condense radial information.
• Angulagram generation: Produces an angulagram as a two-dimensional plot of integrated intensity versus angle.
• Quantitative metrics: Determines stream width, stream linearity, and stream deflection from the angulagram.

Scientific Applications:

• Quantitative MSS evaluation: Enables quantitative assessment of molecular-stream separations via measured stream width, linearity, and deflection.
• Data standardization: Provides a standardized 2D representation (angulagram) for reproducible presentation of MSS data.
• Separation performance assessment: Facilitates evaluation of separation efficiency and precision in biochemistry, molecular biology, and chemical engineering.

Methodology:

Transform MSS intensity and spatial coordinates into a polar coordinate system, integrate intensity along the radius axis to produce an angulagram (integrated intensity versus angle), and extract stream width, stream linearity, and stream deflection.