TopLib

TopLib builds and searches top-down mass spectral libraries for proteoform identification using an efficient spectral representation technique to reduce database size and improve query speed and performance.

Key Features:

• Efficient spectral representation: Reduces database size and improves query speed and performance compared to conventional methods.
• Library building: Constructs top-down mass spectral libraries for proteoform identification.
• Library searching: Searches top-down mass spectral libraries against query spectra for proteoform identification.
• Systematic evaluation framework: Enables evaluation of various spectral representation techniques and scoring functions for top-down spectral clustering and search.

Scientific Applications:

• Proteoform identification: Identifies proteoforms in top-down mass spectrometry (MS)-based proteomics through spectral library search.
• Top-down spectral clustering: Clusters top-down mass spectra using evaluated spectral representation techniques and scoring functions.
• Comparative performance analysis: Demonstrates significantly faster analysis and higher reproducibility in proteoform identification compared to conventional database search methods in top-down MS.

Methodology:

TopLib systematically evaluates various spectral representation techniques and scoring functions for top-down spectral clustering and search.