Trackosome

Trackosome analyzes spatiotemporal dynamics of centrosomes, nuclear envelopes, and cellular membranes during mitosis.

Key Features:

• Centrosome Tracking: Tracks and quantifies centrosome positions and trajectories with high precision to capture migration and separation during early mitosis.
• Membrane Reconstruction: Reconstructs nuclear and cellular membranes from volumetric live-imaging data to provide detailed membrane geometry and dynamics.
• Model-Free Membrane Analysis: Measures membrane movement without relying on circular or elliptical approximations, enabling analysis of irregularly shaped nuclei.
• Nuclear Envelope Fluctuation Quantification: Quantifies dynamic fluctuations of the nuclear envelope to probe mechanical interactions with adjacent cytoskeletal structures.
• Spatiotemporal Metrics Output: Computes metrics describing spatial and temporal relationships among centrosomes, the nucleus, and cellular membranes.

Scientific Applications:

• Centrosome Coordination Studies: Investigates mechanisms driving centrosome separation and positioning during mitosis.
• Nuclear Mechanics Analysis: Identifies modes of oscillation in the nuclear envelope to study cellular mechanical properties.
• Correlation Analysis: Explores correlations between centrosome movements and nuclear/cellular membrane dynamics.

Methodology:

Implemented in MATLAB, it performs centrosome tracking, volumetric membrane reconstruction, model-free membrane movement measurement, nuclear envelope fluctuation quantification, and outputs spatiotemporal metrics.