TriDAMP

TriDAMP visualizes three-way comparisons of metabolite profiles in omics datasets by encoding relative differences with an HSB (hue, saturation, brightness) color-coding scheme.

Key Features:

• Three-Way Comparison Visualization: Enables simultaneous comparison of three datasets to represent differences in metabolite profiles.
• HSB Color Model Utilization: Assigns specific hues to each dataset using the HSB model (e.g., red, green, blue) to distinguish dataset contributions.
• Dynamic Hue Calculation: If two values are identical and one differs, hue corresponds to the differing value; if all three values are distinct, hue is derived from a gradient between the most distant values adjusted by the relative position of the third value.
• Saturation Encoding: Encodes saturation as the amplitude (magnitude) of the numerical difference between the two most distant values.
• Brightness Adjustment: Sets brightness to a maximum by default for clarity and permits modulation of brightness to encode an additional data dimension.

Scientific Applications:

• Large-scale omics studies: Visualizes complex interactions and variations across multiple omics datasets to aid interpretation.
• Metabolite profile comparison: Reveals nuanced differences in metabolite profiles across three conditions or datasets.

Methodology:

Transforms density plots into color-coded visualizations by computing per-point HSB values: assigning dataset hues, deriving hue from gradients between the most distant values with adjustment by the third value, encoding saturation as the amplitude between the two most distant values, and setting or modulating brightness.