TRUST4

TRUST4 reconstructs immune receptor repertoires of αβ and γδ T cells and B cells from bulk and single-cell RNA-sequencing (RNA-seq) data.

Key Features:

• Format Compatibility: Supports FASTQ and BAM file formats for sequencing data input.
• Speed and Sensitivity: Engineered for increased speed and sensitivity, enabling assembly of longer sequences including full-length receptor repertoires.
• Single-Cell RNA-seq Capability: Calls repertoire sequences from single-cell RNA-seq (scRNA-seq) data without requiring V(D)J enrichment.
• Platform Compatibility: Compatible with SMART-seq and 5' 10x Genomics sequencing platforms.

Scientific Applications:

• T and B cell diversity analysis: Facilitates detailed characterization of αβ/γδ T cell and B cell receptor diversity from RNA-seq data.
• Immune response investigation: Supports analysis of immune responses by profiling receptor repertoires.
• Vaccine development research: Aids studies that require assessment of repertoire changes relevant to vaccine responses.
• Autoimmune disease research: Enables repertoire profiling relevant to autoimmune disease studies.
• Functional repertoire reconstruction: Enables reconstruction of full-length receptor sequences to inform analyses of immune cell functionality.

Methodology:

Applies advanced computational techniques to process bulk and single-cell RNA-seq data efficiently and assemble immune receptor sequences with high accuracy.