twoddpcr

twoddpcr is a R/Bioconductor package and Shiny app that enables the analysis of Droplet Digital PCR (ddPCR) data. ddPCR is a sensitive platform to quantify specific nucleic acid molecules amplified by PCR. It is useful for detecting rare mutant molecules, such as those present in circulating free tumor DNA obtained from cancer patients. The twoddpcr package and accompanying GUI allow users to group droplets into four clusters and use Poisson statistics to estimate the number of molecules in two-channel ddPCR data. The GUI also allows users to adjust parameters and view results in real time.