UniDec

UniDec performs automated deconvolution, peak detection, and batch processing of intact-protein mass spectrometry (native and denatured) data to quantify and characterize biotherapeutics such as bispecific antibodies and antibody–drug conjugates.

Key Features:

• Fast processing: Handles large, batched intact mass spectrometry datasets to enable rapid analysis.
• Deconvolution and peak detection: Implements deconvolution and peak detection algorithms for accurate mass assignment and quantitation of complex spectra.
• Spreadsheet-driven batch processing: Iterates through a user-defined spreadsheet listing data files and deconvolution and quantitation parameters to perform systematic analysis across multiple datasets.
• Automated reporting: Produces results in spreadsheet format and generates HTML reports summarizing deconvolution, peak detection, and quantitation outputs.
• Customization and extensibility: Allows modification and extension of workflows and calculations to implement customized analyses.

Scientific Applications:

• Bispecific antibody analysis: Measures correct pairing percentage in bispecific antibody datasets for assessment of product heterogeneity and assembly.
• Antibody-drug conjugate (ADC) evaluation: Measures drug-to-antibody ratios (DAR) to quantify conjugation stoichiometry in ADCs.
• Intact protein mass spectrometry characterization: Supports characterization and quantitation of intact proteins from native and denatured MS workflows.

Methodology:

Processes a user-defined spreadsheet listing data files and analysis parameters, and applies deconvolution and peak detection across datasets to generate quantitative outputs and reports.