Unipept CLI

Unipept CLI infers taxonomic origin and functional annotations for peptides from shotgun metaproteomics to characterize the taxonomic composition and functional capabilities of microbial communities.

Key Features:

• Taxonomic analysis: Infers proteins from identified peptides and assigns taxonomic origin using peptide-to-protein mappings.
• Functional analysis: Retrieves Enzyme Commission numbers, Gene Ontology terms, and InterPro entries for peptides to provide functional annotation.
• Visualization generation: Generates visualizations from lists of taxon IDs for downstream analysis.
• Input support: Processes single peptides or sets of peptides derived from shotgun metaproteomics experiments.
• Database-driven mapping: Uses a comprehensive sequence database for peptide-to-protein and taxon assignment.

Scientific Applications:

• Metaproteomic taxonomic profiling: Determining the taxonomic composition of microbial communities from shotgun metaproteomics peptides.
• Functional profiling: Annotating peptides with EC numbers, GO terms, and InterPro entries to infer enzymatic activities and functional capabilities of microbiomes.

Methodology:

Processes identified peptides to infer proteins and assign taxonomic origin using a comprehensive database, and links peptides to Enzyme Commission numbers, Gene Ontology terms, and InterPro entries for functional annotation.