UniTrap gene trap clones

UniTrap gene trap clones compiles and organizes data from 300,602 gene trap clones to produce 28,199 UniTraps—distinct collections of unambiguous insertions within subgenic regions—supporting experimental design and functional analysis of 9,583 trapped mouse genes, including 7,728 human orthologs and 677 disease-associated genes.

Key Features:

• Dataset aggregation: Processes 300,602 gene trap clones sourced from public and private projects to build the resource.
• UniTrap generation: Produces 28,199 UniTraps defined as distinct collections of unambiguous insertions within specific subgenic regions of annotated genes.
• Genome coverage: Contains data for 9,583 trapped mouse genes, representing approximately 42.3% of the mouse gene content, including 7,728 human orthologs and 677 genes implicated in human disease.
• Clone identification and comparison: Identifies and enables comparison of gene trap clones inserting into genes of interest.
• Mutation severity evaluation: Assesses the impact of each trapping event on protein function to inform potential phenotypic consequences.
• Experimental verification support: Supplies information relevant to PCR, RT-PCR, and restriction experiments and to mapping vector insertion positions within genes.

Scientific Applications:

• Targeted experimental design: Selection of gene trap clones for targeted disruption and follow-up experiments.
• Functional genomics: Analysis of protein function consequences from insertional mutations to infer genotype–phenotype relationships.
• Disease gene investigation: Study of genes implicated in human disease via human ortholog mapping and available trapped clones.
• Clone validation and mapping: Experimental confirmation and precise localization of vector insertions to support downstream molecular assays.

Methodology:

Processed 300,602 gene trap clones from public and private sources to identify unambiguous insertion sites and group them into 28,199 UniTraps mapped to subgenic regions of annotated genes.