uSPIM Toolset

uSPIM Toolset provides control, synchronization, calibration, and acquisition capabilities for Selective Plane Illumination Microscopy (SPIM) to enable high-speed volumetric fluorescence imaging and brain-wide calcium activity measurements in live organisms such as larval zebrafish.

Key Features:

• Hardware control and synchronization: Software-driven control and synchronization of microscope components for scanned-laser-beam SPIM implementations.
• MicroManager integration: Integration with MicroManager for device control and image acquisition in SPIM configurations.
• Calibration procedures: Calibration workflows that optimize image acquisition parameters for specific optical designs and hardware configurations.
• Scanned-beam high-speed acquisition: Support for scanned laser beam acquisition enabling volumetric imaging at rates up to 100 planes per second.
• Single-cell resolution imaging: Capability to capture calcium activity at single-cell resolution across the larval zebrafish brain.
• Flexibility to optical and hardware variations: Operates independently of particular optical designs or hardware configurations to accommodate custom SPIM setups.

Scientific Applications:

• Brain-wide calcium imaging: Measurement of calcium activity across the larval zebrafish brain at single-cell resolution.
• Functional imaging with high temporal resolution: Functional fluorescence imaging studies that require rapid volumetric acquisition to capture fast dynamics.
• High-resolution volumetric fluorescence imaging: Volumetric imaging for cellular- and system-level investigations using SPIM.

Methodology:

Implements MicroManager-based device control and synchronization, scanned laser beam acquisition, and calibration procedures to optimize image acquisition for SPIM setups.