Validated GPCR Antibodies

Validated GPCR Antibodies provides a comprehensive dataset of validated anti-GPCR antibodies with selectivity and epitope annotations to support therapeutic-antibody design and investigation of GPCR-targeting autoantibodies.

Key Features:

• Extensive Validation Dataset: Data derive from multiplexed immunoassay testing of over 400 anti-GPCR antibodies sourced from the Human Protein Atlas against a curated library of 215 expressed and solubilized GPCRs spanning all subfamilies.
• Selectivity Insights: Reported outcomes indicate approximately 61% of antibodies were selective for their intended targets, ~11% exhibited off-target binding, and ~28% did not bind any GPCR.
• Epitope Characterization: On-target antibodies preferentially target antigens that are longer, more disordered, and less likely to be buried within the GPCR structure compared to off-target or non-binding antibodies.
• Research Applications: The dataset informs design of therapeutic antibodies with improved selectivity and enables identification of pathological autoantibodies against GPCRs.
• Methodological Rigor: Validation was performed using a multiplexed immunoassay to enable high-throughput evaluation of antibody specificity across GPCR subfamilies.

Scientific Applications:

• Therapeutic Development: Provides validated antibodies and selectivity profiles to support development of anti-GPCR therapies.
• Autoimmune Research: Supports detection and study of autoantibodies against GPCRs to investigate autoimmune pathologies.
• Structural Biology: Epitope characteristic data facilitate structural studies of GPCRs and receptor–ligand interactions.

Methodology:

Validation employed a multiplexed immunoassay testing over 400 Human Protein Atlas anti-GPCR antibodies against a library of 215 expressed and solubilized GPCRs.