vectorstrip

vectorstrip removes vector sequences from the ends of nucleotide sequences to isolate target DNA from cloning vectors for downstream analyses.

Key Features:

• Vector removal: Removes vector sequences from sequence ends to isolate target inserts derived from cloning vectors.
• EMBOSS integration: Operates as part of the European Molecular Biology Open Software Suite (EMBOSS).
• EMBOSS C libraries: Leverages EMBOSS's extensible C programming libraries for implementation.
• ACD-based customization: Supports customization and development via Application Configuration Definition (ACD) files.

Scientific Applications:

• Sequence preparation: Producing vector-free nucleotide sequences for sequence alignment, assembly, and functional annotation.
• Genomic research: Preparing clean DNA sequences for genomic mapping and annotation workflows.
• Transcriptomics: Removing non-target vector sequences to improve transcriptome assembly and analysis.
• Proteomics-related studies: Providing vector-free cDNA sequences to support analyses of protein-coding genes.

Methodology:

Implemented within EMBOSS using its C programming libraries and configurable via Application Configuration Definition (ACD) files.