Vesiclepedia

Vesiclepedia provides a curated compendium of molecular data for extracellular vesicles, including exosomes and microvesicles, cataloguing their lipid, RNA (including exosomal-shuttle RNAs, esRNAs), and protein constituents to support research into EV composition and function.

Key Features:

• Comprehensive Molecular Data: Includes curated entries of lipids, RNAs, and proteins identified in various types of extracellular vesicles.
• Manually Curated Content: Aggregates molecular data and literature annotations through manual curation to ensure data quality.
• Diverse Extracellular Vesicle Classes: Covers multiple EV classes, including exosomes and microvesicles, to reflect molecular diversity across vesicle types.

Scientific Applications:

• Cataloguing EV Composition: Enables analysis and comparison of lipid, RNA, and protein constituents across EV classes.
• Study of RNA Packaging Mechanisms: Supports investigation of exosomal-shuttle RNAs (esRNAs) and mechanisms of RNA transport into exosomes.
• Identification of RNA-Binding Proteins (RBPs): Documents RBPs detected in exosomes, including a reported set of 30 RBPs implicated in RNA–RBP complexes.
• Functional Validation of RBPs: Informs studies using gene silencing of RBPs (HSP90AB1, XPO5, hnRNPH1, hnRNPM, hnRNPA2B1, MVP) that identified MVP as reducing total esRNA levels when silenced.
• Biochemical Interaction Studies: Supports interpretation of experiments showing increased recovery of esRNA from exosomes upon expression of biotinylated-MVP, consistent with MVP–RNA interactions.

Methodology:

Manual curation and aggregation of published molecular data and experimental annotations into a centralized database of extracellular vesicle lipids, RNAs, and proteins.