Vidjil

Vidjil analyzes high-throughput sequencing data of V(D)J recombinations to assemble and track B-cell and T-cell clonotypes for immune repertoire profiling and disease monitoring.

Key Features:

• V(D)J recombination analysis: Performs analysis of V(D)J recombinations from B and T lymphocytes to characterize antigen receptor sequences.
• Clonotype assembly: Aggregates sequencing reads into clonotypes based on V(D)J junction sequences using an algorithm that groups reads by their junctions.
• Clonotype dynamics visualization: Visualizes clonotype frequencies and dynamics over time to support longitudinal studies of immune responses and disease progression.
• RepSeq and FASTQ support: Accepts raw sequencing files (FASTQ) and executes Repertoire Sequencing (RepSeq) analysis workflows.

Scientific Applications:

• Immune repertoire profiling: Characterizes B-cell and T-cell repertoires from high-throughput sequencing data.
• Leukemia monitoring and clinical diagnostics: Monitors clonal expansions in leukemia and supports clinical diagnostic assessments.
• Longitudinal immune response tracking: Tracks clonotype dynamics over time to study immune responses and disease progression.
• Hematology and immunology research: Supports studies of lymphocyte diversity and adaptive immunity in research settings.

Methodology:

Workflow steps explicitly include uploading raw sequence files (FASTQ), executing Repertoire Sequencing (RepSeq) analysis software, visualizing results, and annotating clonotypes.