VING

VING generates visualizations of next-generation sequencing (NGS) coverage across specified genomic regions by processing NGS mapping files and genome annotations.

Key Features:

• Standalone R implementation: Processes NGS mapping files and genome annotations using a standalone R script to produce coverage visualizations.
• Versatile visualization modes: Provides multiple viewing modes including strand-specific views and a heatmap mode for multi-experiment representation.
• Strand-specific views: Displays read directionality to support strand-specific expression analysis.
• Heatmap mode: Represents coverage of multiple experiments simultaneously within a single figure for comparative visualization.
• Publication-ready output: Produces high-quality figures suitable for inclusion in publications.
• Customizable coverage snapshots: Allows specification of any genomic region to obtain detailed coverage signals.

Scientific Applications:

• NGS coverage visualization: Visualizes sequencing coverage across user-specified genomic regions.
• Comparative genomic studies: Facilitates comparison of multiple experiments using heatmap and multi-sample displays.
• Differential and strand-specific expression analysis: Supports assessment of differential expression and strand-specific expression patterns.
• Mapping visualization: Enables precise mapping and visualization of sequencing reads against a reference genome.

Methodology:

Processes NGS mapping files alongside genome annotations using a standalone R script to generate precise coverage snapshots.