ViralORFeome

ViralORFeome facilitates cloning and management of viral open reading frames (ORFs) for high-throughput functional studies using the Gateway(R) recombination-based cloning system.

Key Features:

• Database and ORF management: Centralized storage and management of viral ORF sequences and associated clone metadata.
• Primer design: Design of ORF-specific cloning primers for precise amplification of target sequences.
• Sequence validation: Validation of sequence integrity for generated constructs to ensure accurate clones.
• Variant and mutant management: Handling and tracking of ORF variants and mutants to accommodate emerging viral strains.
• Established ORF collections: Access to curated collections of virus ORFs and corresponding plasmid constructs.
• Proteome-scale expression testing: Generation and documentation of plasmid constructs that have been tested for heterologous protein expression at proteome scale.
• Gateway(R) compatibility: Support for recombination-based cloning workflows using Gateway(R) technology.

Scientific Applications:

• Functional characterization: Enabling large-scale determination of viral protein functions and activities.
• Protein-protein interaction mapping: Supporting identification of binding partners and interaction networks of viral proteins.
• Evolutionary analysis: Facilitating study of viral evolution through analysis and comparison of ORF variants and mutants.
• Antiviral target discovery: Providing cloned ORFs and expression data to support development of antiviral strategies targeting specific viral functions.

Methodology:

Includes ORF-specific primer design, sequence validation of constructs, management of ORF variants and mutants, storage of curated ORF collections, and generation/documentation of plasmid constructs compatible with Gateway(R) recombination-based cloning.