VirAmp

VirAmp performs semi-de novo assembly of viral genomes from extremely high-coverage next-generation sequencing (NGS) data to enable accurate reconstruction and analysis of viral genomes.

Key Features:

• Semi-de novo assembly: Performs semi-de novo assembly of viral genomes.
• High-coverage NGS support: Processes extremely high-coverage next-generation sequencing (NGS) data for viral genome reconstruction.
• Integration of computational tools: Integrates a suite of existing computational tools and techniques into a cohesive workflow.
• Automated best practices: Automates best-practice genome assembly and related analyses.
• Scalability across strains: Handles high-coverage sequence data across numerous viral strains.
• Validation: Methodology and workflow were tested using human herpes simplex virus (HSV) datasets.

Scientific Applications:

• Viral genome assembly: Reconstruction of viral genomes from high-coverage NGS data.
• Sequencing data analysis: Analysis and interpretation of high-coverage viral sequencing datasets.
• Comparative viral studies: Comparative analysis across numerous viral strains for virology research.

Methodology:

Semi-de novo assembly implemented as a multi-step process that integrates a suite of existing computational tools and techniques and was tested on human herpes simplex virus (HSV) datasets.