WALTER

WALTER analyzes terminal restriction fragment (TRF) scans to quantify telomere length and perform statistical evaluation of TRF-derived intensity profiles.

Key Features:

• ScanToIntensity: Converts selected areas of TRF scans into intensity profiles.
• IntensityAnalyser: Processes TRF-derived intensity profiles to generate visual summaries and perform statistical analyses.
• TRF processing: Provides standardized processing of terminal restriction fragment (TRF) results for reproducible telomere length quantification.
• Visualizations: Produces boxplots and violin plots from intensity profiles to represent telomere length distributions.
• Statistical analysis: Implements in-house statistical evaluation of telomere length data derived from TRF scans.

Scientific Applications:

• Telomere length quantification: Quantifies telomere length from terminal restriction fragment (TRF) scan data.
• Comparative telomere analysis: Enables comparative analysis of telomere length distributions across samples or conditions using statistical tests and visual summaries.
• Genome stability studies: Supports investigations into telomere dynamics and their role in genome stability and related biological processes.
• Method complementarity: Serves as a TRF-based approach complementary to PCR and next-generation sequencing (NGS) methods for telomere analysis.

Methodology:

ScanToIntensity converts selected regions of TRF scans into intensity profiles; IntensityAnalyser processes those profiles to produce boxplots or violin plots and to perform statistical evaluations of telomere length distributions.