wiff2dta

wiff2dta converts ABI WIFF MS/MS data from Applied Biosystems QStar and QTrap 2000/4000 series instruments into .dta files for downstream proteomics identification workflows.

Key Features:

• Performance Efficiency: Extracts spectrum data from ABI WIFF files at least 10% faster than standard tools that read ABI raw formats.
• Batch Processing Capability: Supports batch conversion of multiple WIFF files to .dta to enable high-throughput data processing.
• Integration and Automation: Operates as a stand-alone application and via command-line execution for integration into automated proteomics pipelines.
• WIFF Spectrum Extraction: Directly extracts MS/MS spectra from the proprietary ABI WIFF format and outputs .dta files.

Scientific Applications:

• Mass spectrometry-based proteomics data preparation: Converts raw WIFF MS/MS data to .dta for compatibility with peptide and protein identification algorithms and downstream analysis.

Methodology:

Extracts MS/MS spectrum data from ABI WIFF files and writes .dta output; optimized for faster spectrum extraction and supports batch conversions and command-line execution.