wsGoBioSpace

wsGoBioSpace performs targeted and untargeted analysis of mass spectrometric lipidomic and metabolomic data to enable comprehensive profiling and identification of polar and non-polar lipid species.

Key Features:

• Database Search Capability: Provides an advanced database search functionality for detailed analysis of mass spectrometric data.
• Targeted and Untargeted Analysis: Supports targeted identification and quantification of specified lipid species and untargeted discovery of unknown lipid entities.
• Compatibility with Lipid Extraction Protocols: Compatible with commonly used lipid extraction protocols to integrate with existing laboratory workflows.
• Methodological Integration (UPLC): Integrates with an ultra performance liquid chromatography (UPLC)-based method developed alongside the software for chromatographic separation.
• High-Resolution Accurate Mass Spectrometry: Utilizes high-resolution, accurate mass spectrometry to enhance detection and identification of lipid species.
• Alternative to Shotgun Lipidomics: Provides a chromatographic and database-driven alternative to direct infusion (shotgun) lipidomics.

Scientific Applications:

• Lipidomics in Plant Biology: Applied to plant lipidomics, including profiling Arabidopsis thaliana leaf lipids with over 260 polar and non-polar lipid species reported.
• Method Comparison: Enables methodological comparisons with direct infusion-based shotgun lipidomics to assess accuracy and comprehensiveness of lipidomic studies.

Methodology:

Performs database searches and both targeted and untargeted analyses on mass spectrometric data.