wtdbg2

wtdbg2 assembles genomes de novo from long noisy PacBio and Oxford Nanopore Technologies (ONT) reads to produce consensus sequences for large-genome reconstruction.

Key Features:

• De novo assembly: Performs de novo sequence assembly specifically for long noisy reads.
• Raw-read processing: Processes raw reads directly without prior error correction.
• Consensus construction: Constructs consensus sequences from intermediate assembly outputs.
• Supported technologies: Handles long-read data from PacBio and Oxford Nanopore Technologies (ONT).
• Speed: Achieves throughput reported as 2–17× faster than previously published long-read assemblers such as CANU and FALCON.
• Accuracy and contiguity: Maintains comparable contiguity and base accuracy relative to other long-read assemblers.
• Large-genome capability: Scales to assemble very large genomes, including the human genome and the ~32 Gb Axolotl genome.
• High-throughput scaling: Supports population-scale long-read assembly projects by scaling to high-throughput sequencing datasets.

Scientific Applications:

• Large-genome assembly: Assembly of large and complex genomes such as the human genome and the 32 Gb Axolotl genome.
• Population-scale assembly: Generation of assemblies for population-scale studies using high-throughput PacBio and ONT datasets.
• Genomic research: Rapid production of genome assemblies to support comparative genomics and other genomic analyses.

Methodology:

Performs de novo sequence assembly by processing raw long noisy reads without prior error correction and constructing consensus sequences from intermediate assembly outputs.