zAMP and zAMPExplorer

zAMP and zAMPExplorer enable reproducible and scalable microbiota profiling through amplicon sequence data analysis and interactive visualization and statistical analysis.

Key Features:

• Amplicon sequence data analysis: zAMP processes 16S rRNA gene sequences for bacteria and archaea and ITS sequences for fungi.
• Database processing module: One module processes databases to optimize taxonomy assignment.
• Benchmarking module: A second module benchmarks primers, databases, and classifier performances.
• Interactive visualization and statistical analysis: zAMPExplorer provides quality control, diversity analysis, and statistical testing.

Scientific Applications:

• Microbiota profiling: The toolbox addresses both research and clinical needs in microbiota profiling.

Methodology:

zAMP is implemented as a Snakemake workflow running within Singularity or Docker containers and installable via Bioconda.