zebrafish

zebrafish automates the calculation of heart rate from video recordings to quantify cardiac function in zebrafish larvae.

Key Features:

• Automatic Heart Rate Calculation: Determines heart rate from video recordings of zebrafish larvae using a fully automatic method.
• Advanced Filtering Techniques: Applies filters to identify the heart region within frames, reduce video noise, and mitigate background fluctuations such as shifting.
• Automated Region-of-Interest Selection and Labor Reduction: Automatically selects regions of interest and minimizes manual counting, reducing labor required for cardiac analysis.
• Accuracy and Reliability: Validated against manual heart rate measurements using 32 zebrafish larval cardiac videos recorded at three days post-fertilization, demonstrating high accuracy and reduced uncertainty relative to manual counting.

Scientific Applications:

• Modeling Mammalian Cardiac Physiology and Pathology: Enhances study of heart development and cardiac-related pathogenesis in zebrafish to inform mammalian cardiac physiology and pathology.
• High Throughput Screening: Enables large-scale, automated cardiac phenotyping suitable for high-throughput screening applications.

Methodology:

Capture video recordings of zebrafish larvae (e.g., three days post-fertilization) and process them through an automated system that applies filtering algorithms to isolate heart regions, reduce noise, mitigate background fluctuations, and calculate heart rates without manual selection.