ZebraShare

ZebraShare facilitates sharing of zebrafish mutant line genotype and phenotype data within ZFIN to enable reuse and discovery in zebrafish genetics and developmental biology.

Key Features:

• Structured submission: Captures genotype, phenotype, representative images, captions, and line designations for mutant zebrafish.
• Data integration and curation: ZFIN curators map mutations, incorporate phenotype and mutation information into the ZFIN database, and cross-reference entries across ZFIN resources.
• Indexing and searchability: Submissions are indexed within ZFIN, enabling allele and line-designation searches equivalent to traditionally submitted data.
• Diverse submission types and examples: Accepts phenotypic mutants, mildly phenotypic mutants, and early lethal mutants, including examples such as phf21aa and kdm1a (mild or no apparent skeletal phenotypes with human disease relevance), ctnnd1 (roles during somitogenesis/brain development), and the snu13a/snu13b double knockout (early embryonic lethal affecting muscle formation).

Scientific Applications:

• Resource optimization: Reduces redundant generation of mutant lines by exposing existing genotypes and phenotypes to the community.
• Gene function studies: Enables investigation of gene function and developmental processes using curated phenotype data across stages such as somitogenesis and muscle formation.
• Human disease modeling: Supports identification of zebrafish models relevant to human disease through mutants like phf21aa and kdm1a.
• Reuse and collaboration: Facilitates reuse of underutilized mutant lines and cross-laboratory collaborations by making lines discoverable.

Methodology:

Researchers submit structured genotype/phenotype records and images; ZFIN curators map mutations, incorporate and cross-reference data across ZFIN databases, and index submissions for search.