Anaquin

Anaquin is a tool that uses artificial RNA standards, called 'sequins,' to improve RNA sequencing analysis. Sequins represent spliced mRNA isoforms and aid in measuring sample-specific biases, determining reliable transcript assembly, and quantifying expression. They help navigate the complexity of the transcriptome by providing qualitative and quantitative references, including for cancer diagnosis through fusion gene sequins. Anaquin thereby enhances the understanding and reliability of RNA-seq data.

Topic

Sequencing;Sequence analysis;Data visualisation

Detail

  • Operation: Deposition

  • Software interface: Command-line user interface,Library

  • Language: R

  • License: The 4-Clause BSD License

  • Cost: Free

  • Version name: 2.26.0

  • Credit: -

  • Input: -

  • Output: -

  • Contact: Ted Wong t.wong@garvan.org.au

  • Collection: -

  • Maturity: Stable

Publications

  • Spliced synthetic genes as internal controls in RNA sequencing experiments.
  • Hardwick SA, et al. Spliced synthetic genes as internal controls in RNA sequencing experiments. Spliced synthetic genes as internal controls in RNA sequencing experiments. 2016; 13:792-8. doi: 10.1038/nmeth.3958
  • https://doi.org/10.1038/nmeth.3958
  • PMID: 27502218
  • PMC: -
  • ANAQUIN: a software toolkit for the analysis of spike-in controls for next generation sequencing.
  • Wong T, et al. ANAQUIN: a software toolkit for the analysis of spike-in controls for next generation sequencing. ANAQUIN: a software toolkit for the analysis of spike-in controls for next generation sequencing. 2017; 33:1723-1724. doi: 10.1093/bioinformatics/btx038
  • https://doi.org/10.1093/bioinformatics/btx038
  • PMID: 28130232
  • PMC: -

Download and documentation

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