Afann

Afann (Alignment-Free methods Adjusted by Neural Network) is a software tool that improves the accuracy of alignment-free methods for comparing genome sequences or raw sequencing samples without assembly. Alignment-free methods are faster and more memory-efficient than alignment-based methods. Still, they can overestimate the dissimilarity between sequencing samples and the dissimilarity calculated based on their genomes. This bias can significantly decrease the performance of the alignment-free analysis. Afann addresses this issue by using a neural network to adjust the bias, improving performance on various independent datasets.