Chromagnon

Chromagnon is a tool correct chromatic shifts in three-dimensional fluorescence microscopic images. The Chromagnon algorithm uses a quadrisection phase correlation approach to compute translation, rotation, and magnification from reference images. Chromagnon splits images into small elements to account for small sections and measures the phase correlation between channels and corrects as required with a 3D accuracy of approximately 15 nm.

Topic

Biological imaging;Imaging;Cell biology

Detail

  • Operation: Image analysis

  • Software interface: Library

  • Language: Java;Python

  • License: GNU General Public License v2

  • Cost: Free

  • Version name: 0.91

  • Credit: JSPS KAKENHI, Wellcome Trust Strategic Award.

  • Input: -

  • Output: -

  • Contact: Atsushi Matsuda a.matsuda@nict.go.jp

  • Collection: -

  • Maturity: -

Publications

  • Accurate and fiducial-marker-free correction for three-dimensional chromatic shift in biological fluorescence microscopy.
  • Matsuda A, Schermelleh L, Hirano Y, Haraguchi T, Hiraoka Y. Accurate and fiducial-marker-free correction for three-dimensional chromatic shift in biological fluorescence microscopy. Sci Rep. 2018 May 15;8(1):7583. doi: 10.1038/s41598-018-25922-7. PMID: 29765093; PMCID: PMC5954143.
  • https://doi.org/10.1038/s41598-018-25922-7
  • PMID: 29765093
  • PMC: PMC5954143
  • High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon
  • Kaushik A, et al. miRMOD: a tool for identification and analysis of 5' and 3' miRNA modifications in Next Generation Sequencing small RNA data. miRMOD: a tool for identification and analysis of 5' and 3' miRNA modifications in Next Generation Sequencing small RNA data. 2015; 3:e1332. doi: 10.7717/peerj.1332
  • https://doi.org/10.3791/60800
  • PMID: -
  • PMC: 32628160

Download and documentation

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