Decombinator

Decombinator is a software tool that uses a five-item identifier and finite-state automaton to efficiently categorize and store T-cell receptor (TcR) sequences from high-throughput sequencing data. The tool is able to assign sequences quickly and accurately, even with sequencing errors, and has been tested on both artificial and human TcR-β sequences. Analysis of the human dataset revealed V and J usage bias in the peripheral blood repertoire, and demonstrated the challenge of obtaining a comprehensive description of the T-cell repertoire.