EpiMethylTag

EpiMethylTag integrates ATAC-seq or ChIP-seq with bisulfite conversion to detect chromatin accessibility or transcription factor binding concurrently with DNA methylation on the same DNA fragments.

Key Features:

• Simultaneous detection (M-ATAC and M-ChIP): Integrates ATAC-seq or ChIP-seq with bisulfite conversion to enable concurrent measurement of chromatin accessibility or TF binding and DNA methylation on identical DNA samples.
• Low input and low sequencing depth: Optimized to operate with minimal input material and reduced sequencing depth while preserving the ability to assess methylation and accessibility/TF binding.
• Functional interplay analysis: Enables analysis of the relationship between DNA methylation and chromatin accessibility or transcription factor binding, including effects on regulatory element activation and inverse correlations between methylation and accessibility.

Scientific Applications:

• Epigenetic research: Provides simultaneous chromatin state and DNA methylation data to investigate epigenetic regulation of gene expression.
• Transcription factor binding studies: Allows study of TF interactions with methylated DNA regions, including factors such as CTCF and KLF4.
• Regulatory element activation: Facilitates analysis of regulatory element activation in relation to methylation patterns.

Methodology:

Combines ATAC-seq or ChIP-seq (M-ATAC or M-ChIP) with bisulfite conversion to assess chromatin accessibility or TF binding and DNA methylation on the same DNA fragments.