EpiMethylTag

EpiMethylTag is a method that integrates ATAC-seq or ChIP-seq with bisulfite conversion to simultaneously assess chromatin accessibility or transcription factor binding and DNA methylation on the same DNA sample. This technique is rapid, requires low input, and minimal sequencing depth. EpiMethylTag enables researchers to directly investigate the relationship between regulatory element activation and DNA methylation levels, which are thought to be inversely correlated.

Topic

Epigenetics;ChIP-seq;Methylated DNA immunoprecipitation;Transcription factors and regulatory sites;ChIP-on-chip

Detail

  • Operation: Peak calling;Gene methylation analysis;Bisulfite mapping

  • Software interface: Workflow

  • Language: R,Shell

  • License: Not stated

  • Cost: Free of charge

  • Version name: -

  • Credit: Takeda Multiple Myeloma fellowship, and the National Cancer Center.

  • Input: -

  • Output: -

  • Contact: Jane Skok Jane.Skok@nyulangone.org

  • Collection: -

  • Maturity: -

Publications

  • EpiMethylTag: simultaneous detection of ATAC-seq or ChIP-seq signals with DNA methylation.
  • Lhoumaud P, et al. EpiMethylTag: simultaneous detection of ATAC-seq or ChIP-seq signals with DNA methylation. EpiMethylTag: simultaneous detection of ATAC-seq or ChIP-seq signals with DNA methylation. 2019; 20:248. doi: 10.1186/s13059-019-1853-6
  • https://doi.org/10.1186/S13059-019-1853-6
  • PMID: 31752933
  • PMC: PMC6868874

Download and documentation

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